罗丹明B在3种作物中传导能力研究
Study on the Conductivity of Rhodamine B in Three Plants
魏宏 1李猛 1王振 1周洪友 2赵明敏2
作者信息
- 1. 内蒙古农业大学园艺与植物保护学院,内蒙古呼和浩特 010019
- 2. 内蒙古农业大学园艺与植物保护学院,内蒙古呼和浩特 010019;内蒙古农业大学生物农药创制与资源利用内蒙古自治区高等学校重点实验室,内蒙古呼和浩特 010019
- 折叠
摘要
研究罗丹明B荧光示踪剂在大豆、玉米、烟草幼苗上的传导能力.结果表明经水培处理的植株幼苗地上部均能检测到罗丹明B,其中大豆幼苗真叶部含量较高,玉米幼苗叶鞘中含量高于叶,烟草幼苗茎中含量高于叶.经罗丹明B处理后的植株鲜重与未处理之间无显著差异.同时以烟草幼苗作为模式作物,借助活体成像技术发现叶片和茎分别经质量浓度为15 mg/L和30 mg/L罗丹明B处理后的荧光强度最高.
Abstract
In this work,the Rhodamine B was selected as fluorescent tracer to investigate its conductivity on soybean,maize and tobacco seedlings.The results suggested that Rhodamine B could be detected in the aboveground parts of hydroponic treated plant seedlings,therein,soybean seedlings had the highest content in part of true leaves,the leaf sheath of maize seedlings had a higher content compared with leaves,and tobacco seedlings had a higher content in the stem than leaves.There was no significant difference in fresh weight between Rhodamine B treated plants and untreated plants.And choosing tobacco seedlings as the model crop,the fluorescence intensity was dedected by in vivo ima-ging system.The results showed that the fluorescence intensity was the highest in leaves and stems with treat concentrations of 15 mg/L and 30 mg/L,respectively.
关键词
罗丹明B/植物传导/活体荧光成像Key words
Rhodamine B/Plant transmission/In vivo fluorescence imaging引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据