Genetic Diversity Analysis of Atractylodes Based on Different Genes
In order to explore the genetic diversity and relatedness of Atractylodes,the psbA-trnH,matK and ITS genes of five species of Atrac-tylodes were analyzed.The results showed that there were four psbA-trnH gene-based conversion sites and five inversion sites;there was only one conversion site based on the matK gene in Atractylodes carlinoides,and there were four inversion sites;there were 82 ITS gene-based conversion sites and 15 inversion sites.The GC content of the psbA-trnH gene was 23.6%-24.0%,that of the matK gene was 32.1%-32.5%,and that of the ITS gene was 59.7%-63.8%.Genetic distance analysis showed that the variability ranged from 0 to 0.058 based on the psbA-trnH gene,from 0 to 3.718 based on the matK gene,and from 0.018 to 0.172 based on the ITS gene.The phylogenetic tree analysis showed that based on the psbA-trnH gene Atractylodes japonica and Atractylodes coreana were close relatives,and Atractylodes carlinoides and Carduus nu-tans were close relatives.Based on the matK gene,Atractylodes carlinoides and Carduus nutans were close relatives,and Atractylodes coreana,At-ractylodes japonica,Atractylodes lancea,Atractylodes macrocephala were close relatives.Based on the ITS gene Atractylodes carlinoides and Car-duus nutans were the closest relatives,and Atractylodes coreana,Atractylodes japonica,Atractylodes lancea,Atractylodes macrocephala were close relatives,among which Atractylodes coreana and Atractylodes japonica were closer.In summary,ITS was the strongest recognition ability of se-quences among the three genes and the highest segregation for species of Atractylodes spp.Among them,Atractylodes coreana and Atractylodes japonica indicated closer affinity from all angles,and it was hypothesized that they might have evolved from the same ancestor.It can fill the gap of the molecular status study of Atractylodes spp.in China,and also provide the basis for the identification of Atractylodes spp.in the me-dicinal plant market.
psbA-trnH genematK geneITS geneGenetic diversityAtractylodes DC
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