Establishment of an Efficient in vitro Regeneration System of Bulbel of Chinese chive
In this study,the specific bud on the upper part of the receptacle of Chinese chive plant(called bulbil)found in the field was used as the explant for tissue culture.Through the study of different hormone ratio,the regeneration system of Chinese chive bulbil was established.The results showed that using 10%sodium hypochlorite solution for 7 min had the best disinfection effect and the sterility rate was 82.32%,MS+1.5 mg/L 6-BA+0.4 mg/L 2,4-D was the best medium for adventitious bud induction,and the adventitious bud induction rate was 78.52%.MS+2.0 mg/L 6-BA+0.5 mg/L NAA was the best medium for adventitious bud elongation,and the elongation of adventitious bud reached 86.63%,and 1/2MS+0.5 mg/L IBA was the best rooting medium,the rooting frequency was 88.20%,the survival rate of transplanting was more than 95%.
万方数据
维普
