安徽农业科学2024,Vol.52Issue(24) :59-61,79.DOI:10.3969/j.issn.0517-6611.2024.24.014

刺儿菜内生细菌多样性对镉胁迫的响应

Response of Endophytic Bacterial Diversity in Cirsium arvense to Cadmium Stress

刘佳丽 李明菲 武全伟 陈鉴 陈颖慧 孙潇彤 丁瑞雪 齐磷 赵福强
安徽农业科学2024,Vol.52Issue(24) :59-61,79.DOI:10.3969/j.issn.0517-6611.2024.24.014

刺儿菜内生细菌多样性对镉胁迫的响应

Response of Endophytic Bacterial Diversity in Cirsium arvense to Cadmium Stress

刘佳丽 1李明菲 1武全伟 2陈鉴 3陈颖慧 1孙潇彤 4丁瑞雪 4齐磷 4赵福强4
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作者信息

  • 1. 沈阳大学生命科学与工程学院,辽宁 沈阳 110044
  • 2. 国能宁夏灵武发电有限公司,宁夏灵武 751400
  • 3. 沈阳大学党委统战部,辽宁 沈阳 110044
  • 4. 沈阳大学生物入侵研究中心,辽宁 沈阳 110044
  • 折叠

摘要

[目的]探究不同内生菌群落对镉(Cd)胁迫的响应以及对宿主Cd积累的影响.[方法]以刺儿菜内生菌为研究对象,通过土壤灭菌重塑其群落结构,利用16S rDNA基因高通量测序明确Cd胁迫下不同刺儿菜内生细菌群落结构变化及其对刺儿菜富集特性的影响.[结果]内生菌重塑(ST)处理明显改变了内生菌群落结构,增加了内生细菌群落多样性.Cd胁迫下一些能够促进植物生长的细菌,如鞘氨醇单胞菌属、德沃斯氏菌属成为优势类群.Cd胁迫处理中刺儿菜的生物量显著高于对照组,其中根、叶生物量在Cd胁迫下显著增加(P<0.05).内生菌重塑(ST)处理显著提高刺儿菜Cd含量与Cd累积量(P<0.05).[结论]刺儿菜植株内不同内生细菌群落对Cd胁迫的响应不同,并影响着刺儿菜对Cd的富集能力.

Abstract

[Objective]To explore the response of different endophytic bacterial communities to cadmium(Cd)stress and their impact on host Cd accumulation.[Method]Taking the endophytic bacteria of C.arvense as the research object,the community structure was reshaped through soil sterilization,and high-throughput 16S rDNA gene sequencing was used to clarify the changes in the community structure of different endo-phytic bacteria of C.arvense under Cd stress and its impact on the enrichment characteristics of C.arvense.[Result]Endophytic reshaping(ST)treatment significantly changed the structure of endophytic bacterial communities and significantly increased their diversity.Under Cd stress,some bacteria that could promote plant growth,such as Sphingomonas and Devosia became dominant groups.The biomass of C.arvense in cad-mium stress treatment was significantly higher than that in the control group,with a significant increase in root and leaf biomass under Cd stress(P<0.05).The reshaping of endophytic bacteria(ST)significantly increased the cadmium content and accumulation in C.arvense(P<0.05).[Conclusion]Different endophytic bacterial communities within the C.arvense plant have different responses to Cd stress and affect its ability to accumulate Cd.

关键词

刺儿菜/镉胁迫/内生细菌/多样性

Key words

Cirsium arvense/Cd stress/Endophytic bacteria/Diversity

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出版年

2024
安徽农业科学
安徽省农业科学院

安徽农业科学

影响因子:0.413
ISSN:0517-6611
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