[Objective]To explore the effects of freeze-thaw cycles on the diversity,composition and potential metabolic function of soil bacte-rial community.[Method]The collected soil was treated with different freeze-thaw cycles,we used high-throughput sequencing techniques and Tax4Fun functional prediction to analyze changes in bacterial community diversity and potential metabolic function.[Result]After freeze-thaw treatment,soil physical and chemical properties were significantly different from control group(P＜0.05),the soil bacterial community diversi-ty decreased gradually with the increase of freeze-thaw times,and the freeze-thaw 8 cycles(C3)treatment decreased by 2.54%;Actinobacte-ria dominated(30.91%,32.75%and 33.89%),followed by Proteobacteria and Acidobacteria.A total of 277 functional pathways were found in the experimental soil,and the functional pathways related to metabolism accounted for 64.60%-65.20%of all functions,accounting for the main position.The increase of freeze-thaw cycles not only changed the bacterial community structure,but also affected its metabolic process.[Conclusion]It provided a theoretical basis for studying the metabolic capacity of soil microbial communities in extreme seasonal freezing and thawing environments in northern China.
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