安徽农业科学2024,Vol.52Issue(24) :72-75.DOI:10.3969/j.issn.0517-6611.2024.24.017

微卫星分析广西沙塘鳢群体遗传多样性

Microsatellite Analysis of Genetic Diversity in the Population of Odontobutis obscurus in Guangxi

陈子桂 李华 周大颜 卢飞麟 潘江旺 卢维英 刘羚
安徽农业科学2024,Vol.52Issue(24) :72-75.DOI:10.3969/j.issn.0517-6611.2024.24.017

微卫星分析广西沙塘鳢群体遗传多样性

Microsatellite Analysis of Genetic Diversity in the Population of Odontobutis obscurus in Guangxi

陈子桂 1李华 1周大颜 1卢飞麟 1潘江旺 1卢维英 1刘羚1
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作者信息

  • 1. 广西壮族自治区水产引育种中心,广西 南宁 530031
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摘要

利用10对微卫星分析广西钦州市(QZ)、河池市(HC)及柳州市(LZ)沙塘鳢群体遗传多样性,结果表明:3 个沙塘鳢群体样本均能获得清晰稳定的扩增条带,显示出不同程度的多态性.10个位点有效等位基因数量为0.926 6~4.213 0,平均为2.162 1,期望杂合度为0.106 8~0.843 1,平均为0.475 1,观测杂合度在0.149 1~0.803 4,平均为0.486 8,多态性信息含量在0.081 1~0.598 4,平均为0.331 0.3个群体的有效等位基因数量表现为HC>LZ>QZ,观测杂合度表现为HC>LZ>QZ,群体期望杂合度表现为HC>LZ>QZ,群体多态信息含量表现为HC>LZ>QZ.HC、LZ、QZ 3个不同群体存在中等遗传分化,平均基因分化系数为0.100 3,表明有10.03%的遗传分化来自3 个不同地理群体之间,89.97%的遗传分化来自沙塘鳢内部个体之间.

Abstract

This study used 10 pairs of microsatellites to analyze the genetic diversity of the Odontobutis obscurus population in Guangxi.Clear and stable amplified bands were obtained in samples from three Odontobutis obscurus populations in Qinzhou City(QZ),Hechi City(HC),and Liuzhou City(LZ),showing varying degrees of polymorphism.The effective alleles for 10 loci range from 0.926 6 to 4.213 0,with an average of 2.162 1 and an expected heterozygosity of 0.106 8 to 0.843 1,with an average of 0.475 1,the observed heterozygosity is between 0.149 1 and 0.803 4,with an average of 0.486 8.The polymorphism information content is between 0.081 1 and 0.598 4,with an average polymorphism infor-mation content of 0.311 0.The number of effective alleles in the three populations is HC>LZ>QZ,the observed heterozygosity value is HC>LZ>QZ,the expected heterozygosity value is HC>LZ>QZ,and the population polymorphism information content is HC>LZ>QZ.There is moderate genetic differentiation among the three different populations of HC,LZ,and QZ,with an average gene differentiation coefficient of 0.100 3,indi-cating that 10.03%of genetic differentiation comes from among the three different populations in the field,and 89.97%of genetic differentiation comes from within individuals of the Odontobutis obscurus.

关键词

沙塘鳢/微卫星/遗传多样性分析/遗传分化

Key words

Odontobutis obscurus/Microsatellites/Genetic diversity analysis/Genetic differentiation

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出版年

2024
安徽农业科学
安徽省农业科学院

安徽农业科学

影响因子:0.413
ISSN:0517-6611
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