Inhibition of Fusarium oxysporum Antagonist Cs12 to Saffron Root Rot Under Grape-saffron Intercropping
[Objective]To analyze the microbial community composition and differentially expressed flora in the root soil of saffron mono-cropped soil and grape and saffron intercropping soil.[Method]Root soil was collected in the grape garden of Huzhou Modern Agriculture Dem-onstration Garden in the plot of grape and saffron intercropping and saffron monocropping.We constructed the bacterial resource bank using the RoToR HDA colony high-throughput screening workstation.The beneficial bacteria were screened by isolation and purification of intercropping bacteria and plate confrontation.The incidence of saffron was analyzed to explore the inhibitory effect of beneficial bacteria on saffron root rot.[Result]Through the isolation and purification of intercropping bacteria and plate confrontation,we isolated an antagonistic antibacterial agent of Fusarium oxysporum Cs12.The inoculation experiment showed that Cs12 reduced the incidence index by 50%,and the control effect of CS12 against Fusarium oxysporum was 75%.[Conclusion]We have isolated a newly discovered beneficial bacterium Cs12,which has a good antago-nistic effect against Fusarium oxysporum.The saffron-grape intercropping system can not only solve the problem of saffron rot,but also improve the utilization rate of land resources.This biological control method has certain reference value for the control of soil-borne diseases of other plants,and is helpful for the sustainable development of agricultural industry.
Grape-saffron intercroppingFusarium oxysporum antagonist Cs12Saffron root rotMicrobial community diversity
万方数据
维普
