Research on Tissue Culture Technology of Oreorchis patens in Changbai Mountain
[Objective]To study the tissue culture conditions of Oreorchis patens.[Method]The pseudobulbs of Oreorchis patens in Changbai Mountain were used as research materials to analyze the effects of disinfection treatment,hormone combination and rooting culture on the tissue culture of pseudobulbs of Oreorchis patens in order to screen the best disinfection treatment combination and the optimal medium for different stages of pseudobulbs.[Result]The optimal disinfection treatment of pseudobulbs was 75％alcohol for 45 s+0.1％mercuric chloride for 5 min,the contamination rate of aseptic seedlings was 10.94％,and the survival rate was 87.62％.The best medium for proliferation,differentia-tion and rooting was1/4 MS+1.5 mg/L ZT+0.050 mg/L NAA+20 g/L sucrose+8 g/L agar,pH 5.6,and the proliferation rate was 78.89％,the differentiation rate was 88.34％,and the rooting rate was 83.85％.[Conclusion]This study established the tissue culture and regen-eration system of Oreorchis patens,which can provide technical support and development basis for the protection and breeding of other endan-gered plants in Changbai Mountain area.
万方数据
维普
