筛选Toll样受体激动剂用于羊抗人载脂蛋白A1抗体的制备
Selecting Toll-like Receptor Agonists for the Preparation of Sheep Anti-human Apolipoprotein A1 Antibody
邓碧华 1周宇杰 2尹文竹 3唐欣薇 4梅栋 4徐志伟4
作者信息
- 1. 江苏丘陵地区镇江农业科学研究所,江苏 镇江 212400;江苏省农业科学院动物免疫工程研究所,江苏 南京 210014
- 2. 江苏省农业科学院动物免疫工程研究所,江苏 南京 210014;南京农业大学动物医学院,江苏 南京 210095
- 3. 江苏省农业科学院动物免疫工程研究所,江苏 南京 210014
- 4. 江苏丘陵地区镇江农业科学研究所,江苏 镇江 212400
- 折叠
摘要
为了筛选能够提高羊免疫应答的Toll样受体激动剂,设计针对羊TLR1～TLR10 基因序列的引物,采用PCR方法检测羊体内Toll样受体的表达情况.然后,将人载脂蛋白A1(ApoA1)和HMT13佐剂配伍不同的TLR1～TLR9样受体激动剂,分组免疫湖羊后采集血样,并通过琼脂扩散试验检测抗体效价.结果表明:在湖羊的血液中均可检测到TLR1～TLR10的特异性目的片段.经过3、5、7次免疫后,添加TLR3激动剂的组效价最高,说明TLR3激动剂Ploy(I∶C)免疫增强效果最佳,可作为提高羊疫苗免疫的候选激动剂.
Abstract
In order to select Toll-like agonists that can enhance the immune response in sheep,PCR method was used to design the primers for the gene sequences of TLR1-TLR10 for sheep,and detect the expression of Toll-like receptors in sheep.Subsequently,human apolipopro-tein A1(ApoA1)and HMT13 adjuvant were combined with various commercialized TLR1-TLR9 agonists.Post immunization of Hu Sheep in different groups,blood samples were collected and antibody titers were detected by using agar diffusion test.The results indicated the specific target fragments of TLR1-TLR10 were detected in the blood of Hu Sheep.After 3,5,and 7 rounds of immunization,the titer in TLR3 agonist group was the highest,which suggested that the TLR3 agonist Poly(I∶C)exhibited the best immuno-enhancing effect,so it could be used as a candidate agonist for improving the vaccine immunogenicity in sheep.
关键词
Toll样受体/激动剂/湖羊/ApoA1/免疫应答Key words
Toll-like receptors/Agonist/Hu Sheep/ApoA1/Immune response引用本文复制引用
出版年
2025
国家科技期刊平台
NSTL
维普
万方数据