Selecting Toll-like Receptor Agonists for the Preparation of Sheep Anti-human Apolipoprotein A1 Antibody
In order to select Toll-like agonists that can enhance the immune response in sheep,PCR method was used to design the primers for the gene sequences of TLR1-TLR10 for sheep,and detect the expression of Toll-like receptors in sheep.Subsequently,human apolipopro-tein A1(ApoA1)and HMT13 adjuvant were combined with various commercialized TLR1-TLR9 agonists.Post immunization of Hu Sheep in different groups,blood samples were collected and antibody titers were detected by using agar diffusion test.The results indicated the specific target fragments of TLR1-TLR10 were detected in the blood of Hu Sheep.After 3,5,and 7 rounds of immunization,the titer in TLR3 agonist group was the highest,which suggested that the TLR3 agonist Poly(I∶C)exhibited the best immuno-enhancing effect,so it could be used as a candidate agonist for improving the vaccine immunogenicity in sheep.
万方数据
维普
