Cloning and Expression Analysis of Tobacco HSP18.1 Gene Under Abiotic Stress
The CDS region of HSP18.1 gene was cloned from the sterile seedling of Qinyan No.1,and the recombinant plasmid was construc-ted to induce the expression of the gene protein and optimize the expression conditions.By bioinformatics analysis,the abiotic stress expression of the gene in different tissues was investigated.The results showed as follows:this gene belongs to the HSP20 family,with a total length of 615 bp and a total length of 579 bp for CDS,encoding 192 amino acids,and containing multiple phosphorylation and glycosylation sites,and the secondary structure is mainly random curling;tobacco HSP18.1 protein is homologous to Arabidopsis HSP18.1 protein and interacts with multiple proteins.Phylogenetic results showed that tobacco HSP18.1 was closely related to the fruit,but far related to the millet pepper.The expression of NtHSP18.1 gene was strongly induced in roots,stems and leaves under heat stress,indicating that NtHSP18.1 gene is the key to regulate plant heat tolerance.
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