[Objective]In order to explore the extraction technology of polysaccharide from Lentinula edodes and Morchella esculenta in Shangluo.[Method]Polysaccharides were extracted from Lentinula edodes and Morchella esculenta in Shangnan County by ultrasonic assisted hot water extraction.Through single factor experiment,the effects of solid-liquid ratio,ultrasonic time,ultrasonic temperature and hot water extraction temperature on the extraction rate of Lentinula edodes and Morchella esculenta were compared.The orthogonal experiment was de-signed to obtain the optimum extraction process of Lentinula edodes and Morchella esculenta by ultrasonic-assisted hot water extraction.[Result]The results showed that the extraction rate of lentinan was the highest under the conditions of solid-liquid ratio 1∶60,ultrasonic time 30 min,ultrasonic temperature 40℃and extraction temperature 60℃.Under the conditions of solid-liquid ratio of 1∶50,ultrasonic time of 20 min,ultrasonic temperature of 40℃and extraction temperature of 50℃,the extraction rate of polysaccharide from M.esculenta was the highest.[Conclusion]Ultrasonic assisted hot water extraction of Lentinula edodes and Morchella esculenta polysaccharide is a feasible,effec-tive,economical extraction method.
关键词
超声波辅助提取/提取工艺/多糖/正交试验
Key words
Ultrasonic assisted extraction/Extraction process/Polysaccharide/Orthogonal test
维普
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