Isolation and Identification of Vibrio harveyi from Epinephelus coioides
[Objective]To identify the pathogen that caused deaths of Epinephelus coioides in a farm in Zhanjiang,conducted the dissection of Epinephelus coioides with white raised scabies on skin,and bacterial isolation and purification.[Method]Though biochemical reactions,16S rRNA gene cloning and sequencing,a phylogenetic tree was constructed to identify the dominant strain S1.The growth curve of V.harveyi S1 was plotted by OD600 and plate viable count.According to R2,it would determine the correlation and reliability of its growth curve.[Result]The iden-tification result showed that S1 is one of Vibrio harveyi,the same as V.harveyi hq-V149 converged into one branch.The drug sensitivity test re-sults showed that Vibrio harveyi S1 was sensitive to 12 antibiotics such as norfloxacin,cephalexin,levofloxacin and ciprofloxacin,but not to 12 antibiotics such as penicillin G,amtreonam,streptomycin,cefazolin and cefoperazone.[Conclusion]Vibrio harveyi S1 is the pathogen which caused deaths of Epinephelus coioides in a farm in Zhanjiang.The exponential growth time of S1 is 5-12 hours,and then enters the plateau peri-od.
万方数据
维普
