摘要
为探究MS-222对异育银鲫(Carassius auratus gibelio)麻醉效果、复苏效果以及抗氧化酶活性的影响,采用7 个不同浓度(50、60、70、80、90、100和110 mg/L)MS-222对异育银鲫(5.88±0.78)g进行麻醉,通过记录麻醉分期及其时间、鳃中抗氧化酶活性和相关基因的表达,确定异育银鲫的最适MS-222麻醉浓度和时间.结果表明:在MS-222浓度为60、70、80、90 和100 mg/L时,异育银鲫均可进入麻醉第Ⅰ期和第Ⅱ期,均能在10 min内复苏;浓度低于50 mg/L时,异育银鲫难以进入深度麻醉;浓度高于100 mg/L时,异育银鲫累计死亡率为40%.选择MS-222浓度为60和100 mg/L进行麻醉,进一步分析不同麻醉剂浓度对鱼体的潜在氧化损伤.100 mg/L MS-222深度麻醉异育银鲫(约4 min)后,鳃中GSH活性和MDA含量均显著上升(P＜0.05);HSP70、keap1、PIK3A1、nrf1、MAPK3 和caspase3 的mRNA相对表达量显著上调(P＜0.05);放入清水复苏 6h时,鳃中POD活性显著下降(P＜0.05),caspase3 和LYZ基因表达量显著上调(P＜0.05),异育银鲫逐渐恢复到麻醉前水平;60 mg/L MS-222深度麻醉异育银鲫(24 h)后,鳃中GSH活性上升(P＜0.05);NQO、keap1、PIK3A1、Nrf1和MAPK3基因表达量显著上调(P＜0.05),放入清水复苏1 min后,所测指标与麻醉前差异不显著(P＞0.05).异育银鲫运输过程中适宜的MS-222浓度为60～100 mg/L,科研试验中MS-222较适宜的浓度为100 mg/L.
Abstract
In order to investigate the effects of MS-222 on anesthetic level,recovery rate and antioxidant enzyme activity of Carassius auratus gibelio(5.88±0.78 g),seven different concentrations of MS-222(50,60,70,80,90,100 and 110 mg/L)were set.The optimal anesthesia concentration and time of MS-222 to C.gibelio were determined based on the anesthesia stage and time,activities of antioxidant enzymes in the gill and mRNA expression of antioxidant-related genes.The result showed that C.gibelio could enter the stage Ⅰ and stage Ⅱ of anesthesia while anaesthetized at concentrations of 60,70,80,90 and 100 mg/L,and could recover within 10 min.C.gibelio could not enter deep anesthesia while the anesthetic concentration lower than 50 mg/L,and the cumulative mortality was 40％ while the concentration higher than 100 mg/L.Furthermore,two concentrations of MS-222 at 60 mg/L and 100 mg/L were set,and C.gibelio exposed for different time to study the oxidative damage of MS-222 to gills.Results showed that deep anesthesia with 100 mg/L MS-222(about 4 min),GSH activity and MDA content in gills of C.gibelio were significantly increased(P<0.05).The mRNA relative expression of HSP70,keap1,PIK3A1,nrf1,MAPK3 and caspase3 were significantly up-regulated(P<0.05).The POD activity,and mRNA relative expression of caspase3 and LYZ in the gills were recovered to the basal level after 6 h of recovery.After deep anesthesia with 60 mg/L MS-222(about 24 h),GSH activity in gills increased and POD de-creased significantly(P<0.05).The transcription of genes NQO,keap1,PIK3A1,Nrf1,MAPK3 and caspase3 were significantly up-regulated(P<0.05).After 1 min of recovery within water,there was no significant difference with them before anesthesia.This study suggested that the appropriate concentration of MS-222 in the transport process of C.gibelio is more than 60 mg/L,and the concentration in scientific researches is less than 100 mg/L.
维普
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