Construction and Identification of Cucumber Yeast Two-hybrid cDNA Library Under Biological Stress
A yeast two-hybrid cDNA library of cucumber was constructed by Gateway technology under the stress of Pseudoperonospora cuben-sis and Corynespora cassiicola using the disease resistant inbred line D9320.The results showed that the number of yeast library clones was 350,the titer of library was3.5×107 CFU/mL and the rate of recombination was100% .The length of all the fragments was750-2 000 bp.The aver-age length was more than 1 000 bp.The yeast two hybrid cDNA library of cucumber under the stress of Pseudoperonospora cubensis and Corynes-pora cassiicola was constructed successfully,which could be used for screening interaction proteins of the double-disease resistance mechanism in cucumber.
万方数据
维普
