Identification of Genetic Relationships Among Five Walnut Varieties Based on Phenotypic Traits and ISSR Analysis
[Objective]Select the walnut variety Juglans regia'Yanyuanzao'with the largest cultivation area and the largest production in Si-chuan Province,and explore the genetic relationship of Juglans regia'Yanyuanzao'.[Method]This study selected five walnut varieties,Jug-lans regia'Yanyuanzao',Juglans sigillata'Santai',Juglans sigillata'Yangbi',Juglans regia'Wen185'and Juglans regia'Xinxin2'as re-search materials.The single fruit weight,kernel weight,kernel yield,nut diameter,fruit shape index,seed shell thickness,and kernel composition(crude protein)were measured,and the diversity of phenotypic characters and kernel component of five walnut cultivars were studied by the ANOVA,correlation,clustering and principal component.The genetic diversity of five walnut variety including'Yanyuanzao'were investigated by ISSR molecular marker technology.[Result]The single fruit weight,kernel weight,kernel yield,lateral diameter,transverse diameter,lon-gitudinal diameter,fruit shape index,seed shell thickness,and kernel composition(crude fat and crude protein content)of the five walnut va-rieties showed extremely significant or significant differences(P＜0.01 or P＜0.05).The results of cluster and principal component analysis showed that the five walnut varieties could be clustered into three major classes,with the class Ⅰ being'Wen185',the class Ⅱ being'Xinxin2'and'Yanyuanzao'and'Yangbi',and the class Ⅲ was'Santai'.10 primers with clear bands,bright background,high reproduc-ibility,good polymorphism and high stability were screened by using ISSR molecular markers.The results of polymorphism analysis showed that the mean number of effective alleles of the five walnut varieties was 1.337 0,the mean gene diversity index was 0.193 5,and the mean Shannon information index was 0.285 8.The clustering and principal component analysis showed that'Yanyuanzao'and'Yangbi'were clustered to-gether,indicating that the relatives of them were closer,and'Wen185'and'Xinxin2'were clustered together in the same area,and the'San-tai'was clustered into one category.[Conclusion]Cluster analysis of the fruit phenotypic traits,kernel components and ISSR molecular markers of five walnut varieties showed that,'Yanyuanzao'and'Yangbi'had a close genetic relationship.
万方数据
维普
