红大戟根腐病病原鉴定及其生物学特性
Identification and Biological Characteristics of the Root Rot Pathogen of Knoxia roxburghii
刘春菊 1张磊 2李恒 3董家红 2何霞红 4邱斌2
作者信息
- 1. 云南中医药大学中药学院,云南 昆明 650500;云南农业大学植物保护学院,云南 昆明 650201
- 2. 云南中医药大学中药学院,云南 昆明 650500
- 3. 云南云天化股份有限公司研发中心,云南 昆明 650228
- 4. 西南林业大学,云南 昆明 650244
- 折叠
摘要
[目的]分离、鉴定红大戟根腐病的病原菌及掌握该病原菌的生物学特性.[方法]根据病原菌形态特征,结合ITS-5.8S rDNA、tub2和ACT序列分析,依据柯赫氏法则进行致病性试验.[结果]从接种植株中重新分离出形态一致的病原菌,并将其鉴定为大豆茎点霉(Boeremia exigua).生物学特性研究表明,病原菌最适生长培养基为PDA培养基,最适生长温度为20℃或25℃,最适培养的pH为5;最适碳源是乳糖,最适氮源是硝酸钾.[结论]这是B.exigua在中国引起红大戟根腐病的首次报道;掌握其生物学特性,为红大戟根腐病的防治奠定理论基础.
Abstract
[Objective]In order to isolate and identify the pathogen of root rot of Knoxia roxburghii and master the biological characteristics of the pathogen.[Method]According to the morphological characteristics of the pathogen,combined with ITS-5.8S rDNA,tub2 and ACT se-quence analysis,the pathogenicity test was carried out according to Koch's Rule.[Result]The morphologically consistent pathogen was re-isolated from the inoculated plants and finally identified as Boeremia exigua.The studies of biological characteristics showed that the optimum growth medium of pathogen was PDA,the optimum growth temperature was 20℃or 25℃,and the optimum pH was 5.The optimum carbon source was lactose,and the optimum nitrogen source was potassium nitrate.[Conclusion]This is the first report of B.exigua causing root rot of K.roxburghii in China.Mastering its biological characteristics will lay a theoretical foundation for the prevention of root rot of K.roxburghii.
关键词
红大戟/根腐病/大豆茎点霉/鉴定/生物学特性Key words
Knoxia roxburghii/Root rot/Boeremia exigua/Identification/Biological characteristics引用本文复制引用
出版年
2025
国家科技期刊平台
NSTL
维普
万方数据