Determination of 10 Food Additives in Fresh Fruits by Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry
[Objective]To establish a method for the determination of various additives in fruits such as waxberry and mulberry using ultra-high performance liquid chromatography triple quadrupole tandem mass spectrometry.[Method]The pre-treatment of the sample was carried out using acetonitrile methanol water solution(V∶V∶V,1∶1∶1)as the extraction reagent.After shaking and ultrasonic extraction,the sample was diluted and made to volume with pure water.Using 0.22 μm filter membrane filters the samples.Ultra-high performance liquid chromatog-raphy-triple quadrupole tandem mass spectrometry was used for analysis and detection under multi-reaction monitoring(MRM).The separation and analysis were performed on a Waters Atlantis T3 C18(2.1 mm×150 mm,5 μm),quantitative with external standard method.[Result]Within the range of 10.59-3 260.67 ng/mL,the linear relationship of 10 additives was good(R2≥0.993 8),the detection limit of the method was 0.02-1.50 mg/kg,and the quantitative limit was 0.25-4.00 mg/kg.The average recovery rates of 10 additives were 85.21%-108.92% between 2,5 and 10 times the LOQ concentration,and the RSD was 0.74%-9.63% .[Conclusion]The established method has the advantages of simple pretreatment,high sensitivity,good accuracy and strong repeatability,and can be applied to the analysis and detection of additives in fruits such as waxberry and mulberry.
Food additivesFresh fruitsUltra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry
万方数据
维普
