[Objective]To understand the cold and heat resistance of different varieties of Hippeastrum rutilum,and provide theoretical support for the breeding,introduction,and production of Hippeastrum rutilum in China.[Method]Twenty one different varieties of Hippeastrum rutilum,including Gewasi,were selected and subjected to high and low temperature stress treatment in a light culture box.On the 8th day of treatment,relevant indicators of the control group and treatment group of the 21 varieties were measured,including SOD,MDA,POD,ROS,aboveground fresh weight,aboveground dry weight,chlorophyll content,and other indicators.[Result]Under low temperature stress,each index could be di-vided into four principal components,with a cumulative contribution rate of 72.560%.Under high temperature stress,the cumulative contribu-tion rate of the four principal components was 68.804%.Among the 21 varieties of Zhudinghong,the highest cold tolerance D value was from Exotic Star(0.586 3),and the lowest was from Fairy(0.113 9);Ferrari had the highest heat resistance ranking with a D value of 0.654 6,while Jade had the lowest(0.249 8).[Conclusion]Among the 21 germplasm resources of Hippeastrum rutilum,Exotic Star had the strongest cold re-sistance,Ferrari had the strongest heat resistance,Fairy had the weakest cold resistance,Jade had the weakest heat resistance,and Exotic Star had strong comprehensive cold and heat resistance.