Changes of Hematological Parameters in Peripheral Blood of Rana Nigromaculata Infected with Nyctotherans
Rana nigromaculate is one of the most important species of frog raised in China in recent years,which has high ecological and economic value.Nyctotheran is one of the common parasites in the intestinal tract of frogs.The morphometrics and microstructure of peripheral blood cells of Rana nigromaculate infected by Nyctor-therans were studied by means of microscopic cell counting and routine wright's staining.Wright's staining showed that parasite infection had no significant effect on blood cell morphology and structures.Cell counts showed that the number of erythrocytes in the infected group was extremely significantly decreased(P<0.01),and the number of leukocytes and thrombocytes was significantly increased(P<0.05).The classified count in leu-kocytes indicated that the number of lymphocytes,neutrophils and eosinophils increased extremely significantly in the infected group compared with the uninfected group(P<0.01),while there was no significant difference in the number of monocytes and basophils.According to the results of this study and existing literature reports,the changes in the number of erythrocytes and eosinophils can be used as important indicators for the detection of par-asitic infection.
Rana nigromaculateNyctotheransblood cellspercentage of leucocytes