Genomic characteristics of Listeria monocytogenes isolated in Huainan City from 2021 to 2022
Objective To understand the molecular types,virulence genes and drug resistance genes of Listeria monocyto-genes,and to provide scientific basis for prevention and control of it.Methods 10 strains of Listeria monocytogenes isolated in Huainan City from 2021 to 2022 were subjected whole genome sequencing.The molecular types of strains were analyzed online through BIGSdb database,virulence genes and drug resistance genes were analyzed by bioinformatics analysis soft-ware.Results Among 10 Listeria monocytogenes strains,9 were isolated from food and 1 was isolated from a clinical pa-tient.They were divided into 6 sequences types(ST),ST8(50％)was dominant,and the clinical isolates were ST3,which was different from the food isolates.All strains carried virulence islands LIPI-1 and LIPI-2,and only ST3 strains carried LI-PI-3 with the highest number of detected virulence genes.Four strains(ST3,ST1032,ST87,ST91)carried vip virulence genes,all strains carried FosX,mprF,lin,and norB resistance genes.Conclusion The strains of Listeria monocytogenes in Huainan City carried various molecular types,virulence genes and drug resistance genes.Special attention should be paid to strains carrying vip genes.The clinical isolates were highly virulent and different from food isolates,so the monitoring of food samples should be strengthened.
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