In vivo biocompatibility studies of nitrofuran antibacterial bone cement
Objective To study the in vivo biocompatibility of nitrofuran antimicrobial bone cement.Methods Twenty-one C57 mice were randomly divided into groups and injected with commercially available PMMA bone cement extract(concentrations of 40%,80%and 100%),p(10%NFMA-co-MMA)bone cement extract(40%,80%and 100%,respectively)and normal saline(0.9%sodium chloride solu-tion)(7 groups,n=3).The changes of general state and body weight of mice were observed.The liver and kidney of mice were stained histologi-cally to observe whether liver and kidney cells had degeneration and necrosis.Six New Zealand rabbits were selected for back muscle implanta-tion experiments(2 groups,n=3),and the muscle tissues around the samples were histologically stained,and the local tissue reactions were ob-served after two weeks and four weeks of implantation.Results All mice did not show any adverse reactions and none of the mice died.All mice injected with p(10%NFMA-co-MMA)bone cement extract had an increased mass compared with the precursor injection,and the in-crease was not statistically significant compared with that of the control group(P>0.05).Among them,the body mass increase of mice in the 40%PMMA extract group was the most obvious,and the increase in body mass on the third day was(3.56±2.52)g,and the increase was not sta-tistically significant compared with that of the control group(P>0.05).Mouse liver and kidney cells injected with p(10%NFMA-co-MMA)bone cement extract did not show obvious degeneration or necrosis.After implantation of p(10%NFMA-co-MMA)bone cement,a large num-ber of inflammatory cell immersion was observed in the surrounding tissues of the sample at week 2,and the inflammation was reduced at week 4,with the same results as after PMMA bone cement implantation.Conclusions Compared with the PMMA bone cement used in clinical prac-tice,non-leaching antibacterial bone cement p(NFMA-co-MMA)does not cause significant toxic reactions in animals,indicating that p(NFMA-CO-MMA)bone cement has good biocompatibility in animals.
NETL
NSTL
万方数据
维普
