Study on relationship between serum miR-149-5p expression level and condition and prognosis in patients with acute cerebral infarction
Objective To explore the relationship between serum miR-149-5p expression levels and the condition and prognosis of patients with acute cerebral infarction(ACI).Methods From March 2019 to March 2020,124 confirmed ACI patients treated in Baoding No.1 Central Hospital were selected as the research subjects,according to the severity of the condition,they were grouped into the mild group(42 cases),moderate group(46 cases),and severe group(36 cases);according to the follow-up results,all patients were divided into a poor prog-nosis group(40 cases)and a good prognosis group(84 cases);124 healthy individuals who came to the hospital for physical examination during the same period were selected as the control group.Fluorescence quantitative Polymerase Chain Reaction(PCR)method was applied to measure the expression level of miR-149-5p in serum;Pearson method was applied to analyze the correlation between serum miR-149-5p level and the severity of the disease;logistic regression analysis was performed to analyze the influencing factors of patient prognosis.The ROC curve was used to analyze the diagnostic value of serum miR-149-5p level in poor prognosis.Results The serum miR-149-5p level in the diseased group was(0.90±0.29),which was lower than that in the control group(P<0.05);as the severity of the disease increases,the level of miR-149-5p showed a decreasing trend(P<0.05);the serum miR-149-5p level in the poor prognosis group(0.52±0.17)was obviously lower than that in the good prognosis group(1.08±0.35)(P<0.05);age≥70 years old,location of cerebral infarction in the brainstem and extensive cerebral infarc-tion were the risk factors that affected the prognosis of patients with ACI,while elevated miR-149-5p was a protective factor that affected the prognosis of patients with ACI(P<0.05);the ROC curve results showed that the area under the curve for diagnosing poor prognosis in ACI pa-tients with serum miR-149-5p was 0.873,the cutoff value was 0.768,the sensitivity was 85.00%,and the specificity was 76.19%.Conclu-sion The serum miR-149-5p level is down-regulated in patients'with ACI,and the patient´s condition deteriorates.MiR-149-5p is a pro-tective factor for prognosis,and miR-149-5p has certain diagnostic value in poor prognosis in patients with ACI.
Acute cerebral infarctionMiR-149-5pPrognosisBrain stemLarge area cerebral infarction