首页|HPLC法测定桂枝茯苓丸中丹皮酚的含量

HPLC法测定桂枝茯苓丸中丹皮酚的含量

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目的 测定成药桂枝茯苓丸中丹皮酚的含量.方法 采用kromasilODS-C18柱(4.6 mm×150 mm,5 μm),乙腈-水(30: 70)为流动相,流速为1.0 ml·min-1,检测波长为274 nm,柱温25℃.结果 丹皮酚在5.02~ 50.20 mg·L-1的范围内线性关系良好;平均加样回收率为96.92%.结论 该测定方法操作简单,结果准确,重复性好,可以用于控制质量.
Determination of paeonol in guizhifuling pills by HPLC
Aim To establish an HPLC method for determining paeonol in guizhifuling. Methods The determination was conducted on a kromasil C18 column(4.6 mm × 150 mm,5μm)with acetonitrile-water (30:70) as a mobile phase. The flow rate was 1.0 ml·min-1, with detection wavelength at 274 ran at a room temperature of 25℃. Results Paeonol showed good linear relationship in the range of 5.02~50.20 mg·L-1.The average recovery was 96.92%. Conclusion This simple, accurate and reproducible method can be used for the quality control.

guizhifulingpaeonolHPLC

寇婉青、开伟华、王蓉、方玲、刘婧

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安徽中医学院药学院·安徽省高校现代中药重点实验室,安徽,合肥,230031

桂枝茯苓丸 丹皮酚 HPLC

2009

安徽医药
安徽省药学会

安徽医药

CSTPCD
影响因子:1.941
ISSN:1009-6469
年,卷(期):2009.13(5)
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