黑果小檗总黄酮含量测定及体外抗氧化活性研究
Determination of total flavonoids and study on the antioxidation abiltity of berberis heteropoda schrenk in vitro
LI Li SUN Ying-ping LI Si-dong
摘要
目的 测定黑果小檗各部位中总黄酮含量并评价其体外抗氧化活性,为有效利用黑果小檗资源提供参考.方法 采用紫外分光光度法测定黑果小檗中总黄酮的含量,并通过DPPH、羟自由基和氧自由基清除法评价黑果小檗提取物的抗氧化活性.结果 黑果小檗果实中总黄酮含量较高;其次为茎皮及根皮;果实提取液的抗氧化活性较强,茎皮次之.结论 黑果小檗提取物具有一定的抗氧化能力,本研究可为天然抗氧化剂的开发提供依据.
Abstract
Objective To determine the content of total flavonoids in different parts of Berberis heteropoda Schrenk and to study the an-tioxidant activity so as to provide references for efficient utilization of the herbal plant. Methods We determined the total flavonoids of Berberis heteropoda Schrenk by UV-Vis spectrophotometry, evaluated the antioxidant activity of the extracts from different parts of Berberis heteropoda Schrenk in scavenging DPPH radicals, hydroxy radicals and oxygen radicals. Results The contents of total flavonoids were richer in fruits of Berberis heteropoda Schrenk than in stem bark and root bark. In addition, the extracts from the fruits of Berberis heteropoda Schrenk showed higher antioxidant activity than the stems. Conclusion The extracts of Berberis Heteropoda Schrenk show antioxidant capacity,thus can provide a basis for the development of natural antioxidants.
关键词
黑果小檗/总黄酮/抗氧化活性Key words
berberis heteropoda schrenk/total flavonoids/antioxidant activity引用本文复制引用
基金项目
新疆维吾尔自治区科技重大专项(201130105-2)
乌鲁木齐市科学技术计划项目(G121110002)
出版年
2013
NETL
NSTL
维普
万方数据