Establishment and application of gastric cancer cell strain MKN-45 xenograft model of nude mice
Objective To establish an ideal nude mice model beating gastric cancer cell line MKN-45 , and to observe the sensitivity of the xenograft for anti-cancer drags. Methods Nude mice were injected with 5×106 MKN-45 gastric cancer cells in the right flank fat pad. The tumor growth and histopathological features were observed ,and the expression of Ki-67, E-cadherin and vimentin of tumor were detected by immunohistochemistry. After tumor cell inoculation for two weeks , tumor-bearing mice were divided into chemotherapy medi -cation group with 1 mg·kg-1 paclitaxel combined 5 mg·kg-1 fluorouracil and control group with saline (n = 6). The drags were given once per week for consecutive three weeks . Tumor growth and pathological changes were compared between two groups . Results Inoculated 12 mice were successfully tumor-bearing, and had an early tumor-forming time and consistent tumor size and shape ,and keep the important biological characteristics of primary tumor with high-expression vimentin and low-expression E-cadherin. After clinical anti-cancer drag paclitaxel and fluorouracil combined treatment for three weeks , compared with control group , the tumor growth was slowing down , tumor volume was significantly inhibited , Ki-67 expression and neovascularization reduced and cell apoptosis increased in chemotherapy group. However,the expression of vimentin and E-cadherin did not change significantly between two groups . Conclusion The nude mice model bearing gastric cancer cell line MKN -45 is successfully established, which provides an ideal tool for the research of gastric cancer pathogenesis and anti -cancer therapeutic pharmacology .
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