Study on the protective effect and mechanism of paeoniflorin on liver tissue damage caused in HBV-infected mice
Objective To investigate the role and its mechanism of paeoniflorin(PF)on liver tissue injury in HBV-infected mice.Methods From June 2022 to January 2023,40 C57BL/6 mice were divided into Sham group,HBV group,5 mg/kg PF group and 10 mg/kg PF group by randomization,with 10 mice in each group.Except for the control group,the mice in the other groups were injected with recombinant adenovirus vector through tail vein to establish an AAV8-1.3 HBV infection model rat.From 24 hours before model-ing to 5 weeks after modeling,mice were given different doses of PF solution or equal volume solvent by gavage every day.Subsequent-ly,the levels of aspartate aminotransferase(AST)and alanine aminotransferase(ALT)in serum of mice were detected by biochemical analysis.The levels of HBV serum markers(HBeAg)and hepatitis B surface antigen(HbsAg)were detected in serum by enzyme-linked immunosorbent(ELISA)assay.Hematoxylin-eosin(HE)staining was used to detect the pathological changes of liver tissue.Masson staining was used to detect the fibrosis of liver;the levels of lactate dehydrogenase(LDH),interleukin-1β(IL-1β)and interleukin-18(IL-18)in liver tissue of mice were detected by ELISA assay.The apoptosis of liver cells in mice was detected by terminal deoxynucleo-tidyl transferase-mediated dUTP nick end labeling(TUNEL)method.Western blotting was used to detect the pyroptosis-related protein expression of nuclear factor-κB(NF-κB)NF-κB,nod-like receptor protein 3(NLRP3),and gasdermins D(GSDMD)in liver.Results Serum AST(44.89±7.82)U/L,ALT(43.13±5.72)U/L,HBeAg and HbsAg were not expressed in Sham group.The values of AST,ALT,HBeAg and HbsAg in HBV group were(98.76±12.21)U/L,(75.38±1.23)U/L,(52.22±7.15)U/L and(67.33±9.25)U/L,respectively,which were significantly increased in HBV group(P<0.05).Compared with Sham group,the morphology of liver cells in HBV group was significantly changed,inflammatory cell infiltration was obvious,and a large number of liver fibrosis appeared.LDH,IL-1β and IL-18 in HBV group,were significantly higher than those in HBV group.Cell apoptosis in HBV group was increased than those in Sham group,and the expression of NF-κB,NLRP3,GSDMD in HBV group were significantly higher than those in Sham group(P<0.05).Com-pared with HBV group,the levels of AST,ALT,HBeAg and HbsAg in serum of 5 mg/kg PF group and the 10 mg/kg PF group were sig-nificantly decreased(P<0.05).The pathological damage of liver tissue was significantly alleviated,and the degree of liver fibrosis was improved.The levels of LDH,IL-1β and IL-18 in liver tissue were significantly decreased,the cell apoptosis was markedly decreased,and the pyroptosis-related protein expression of NF-κB,NLRP3 and GSDMD was significantly down-regulated(all P<0.05),and these differences between 5 mg/kg PF group and 10 mg/kg PF group were statistically significant(P<0.05).Conclusion PF alleviates liver tissue injury caused by HBV infection by regulating NLRP3-mediated pyroptosis,and PF has a protective effect on liver tissue damage caused by HBV infection in mice.
万方数据
维普
