KIAA1522 promotes triple negative breast cancer metastasis by activating the Wnt signaling pathway
Objective To analyze the mechanism of KIAA1522 activating Wnt signaling pathway and promoting tumor cell metasta-sis in triple negative breast cancer(TNBC).Methods The study was conducted from January 2021 to October 2022,36 cases of triple negative breast cancer treated surgically at Tangshan People's Hospital were collected.TNBC was assigned into two groups according with lymph node metastasis(metastatic group)and without lymph node metastasis(non-metastatic group).The relative expression levels of KIAA1522,IQGAP1 and β-catenin proteins and mRNA in each group were detected by Western blotting and RT-qPCR.The interac-tion of β-catenin respectively with KIAA1522 and IQGAP1 were detected by Co-Immunoprecipitation.Results The relative expres-sion levels of KIAA1522 protein,IQGAP1 protein and β-catenin protein in the metastatic group(0.37±0.05,0.28±0.02,1.50±0.08)were significantly higher than those in non-metastatic group(0.27±0.05,0.25±0.05,1.05±0.02)(P<0.05).The relative expressions of KIAA1522 mRNA and IQGAP1 mRNA in the metastatic group(0.95±0.03,1.08±0.10)were significantly higher than those in non-met-astatic group(0.73±0.05,0.99±0.12)(P<0.05)and they were positively correlated(r=0.55,P<0.05),the relative expression of β-catenin mRNA in the two groups was not statistically significant.Co-immunocoprecipitation showed no interaction between KIAA1522 protein and β-catenin protein in TNBC,in contrast,IQGAP1 protein co-precipitated with β-catenin protein.Conclusion KIAA1522 activates Wnt signaling pathway and promotes the metastasis of TNBC,the mechanism may be related to upregulating of IQGAP1 mRNA,and then overexpression IQGAP1 promotes the accumulation of β-catenin proteins and the binding of protein complexes.
Triple negative breast neoplasmsWnt signaling pathwayKIAA1522IQ motif containing GTPase activating protein 1β-cateninCo-Immunocoprecipitation
万方数据
维普
