LncRNA-BBOX1-2 promotes the occurrence and progression of gastric cancer by regulating FGFR1
Objective To investigate the effect of long non-coding RNA(lncRNA)BBOX 1-2 on the development mechanism of gas-tric cancer(GC)by regulating fibroblast growth factor receptor 1(FGFR1).Methods Tumor tissues and corresponding normal tissues adjacent GC of 30 patients who underwent radical gastrectomy in Luwan Branch of Ruijin Hospital of Shanghai Jiao Tong University School of Medicine from 4,2017 to 1,2019 were retrospectively selected as the study objects,and real-time PCR was used to detect the expression of lncRNA-BBOX1-2 and FGFR1.After SGC-7901 cells transfected with si-linc-BBOX1-2,the changes in tumor cell biolog-ical function and FGFR1 expression by a series of biological function experiments of western blotting,MTT,Transwell,cell scratches,and plate cloning were detected.Results Both lncRNA-BBOX1-2(3.68±0.58 vs.1.15±0.11)and FGFR1(4.26±0.71 vs.1.19±0.18)were overexpressed in GC tissues than those in adjacent normal tissues(P<0.05).si-linc-BBOX 1-2 transfection of SGC-7901 cells showed downregulation of FGFR1 expression and significantly decreased cell viability,migration,invasion and viability.Conclusion LincRNA-BBOX 1-2 can mediate the proliferation,apoptosis,migration and invasion of GC cells by regulating FGFR1 expression,which may provide new targets and potential biological markers for the treatment of GC.
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维普
