Correlation analysis between changes of serum lncRNA ANRIL and BDNF levels and thromboembolism in patients with nephrotic syndrome
Objective To investigate the correlation between the changes of long non-coding RNA(lncRNA)antisense non-coding RNA in the INK4 locus(ANRIL)and brain-derived neurotrophic factor(BDNF)levels and thromboembolism(TE)in patients with ne-phrotic syndrome(NS).Methods Two hundred and twenty NS patients who were hospitalized in Chengde Central Hospital from March 2019 to October 2021 were selected as the study subjects,including 102 NS patients with TE as the TE group,118 NS patients without TE as the NS group,and 100 healthy persons in the same period were regarded as the control group.Real-time fluorescence quantitative PCR(qRT-PCR)was applied to measure the serum lncRNA ANRIL level of the subjects;the serum BDNF level of the sub-jects was detected by enzyme-linked immunosorbent assay(ELISA);automatic biochemical analyzer was applied to detect relevant bio-chemical indicators;Pearson correlation was applied to analyze the correlation between serum lncRNA ANRIL,BDNF levels and bio-chemical indicators;multivariate Logistic regression was applied to analyze the influencing factors of TE in NS patients;the predictive value of serum lncRNA ANRIL and BDNF on the occurrence of TE in NS patients was analyzed by using the Receiver operating Char-acteristic curve(ROC curve).Results Compared with the control group,the serum lncRNA ANRIL level in NS group and TE group was significantly increased(2.54±0.43 vs.5.82±1.34,8.35±2.17).The level of BDNF was significantly decreased[(32.77±8.25)µg/L vs.(24.49±4.58)µg/L,(18.63±3.62)µg/L](P<0.05).Compared with NS group,the serum lncRNA ANRIL level in TE group was sig-nificantly increased,and the serum BDNF level was significantly decreased(P<0.05).The levels of uric acid,serum creatinine,lactate dehydrogenase,blood urea nitrogen,and urine protein in the control group,NS group and TE group were gradually increased,while the levels of serum albumin,and hemoglobin were gradually decreased(P<0.05).Pearson correlation analysis showed that the level of se-rum lncRNA ANRIL was positively correlated with the levels of uric acid,serum creatinine,lactate dehydrogenase,blood urea nitrogen and urine protein in NS patients with TE,and negatively correlated with the levels of serum albumin and hemoglobin(P<0.05);the lev-el of serum BDNF in NS patients with TE was negatively correlated with the levels of uric acid,creatinine,lactate dehydrogenase,blood urea nitrogen and urine protein,and positively correlated with the levels of serum albumin and hemoglobin(P<0.05).Multivariate Logis-tic regression analysis showed that lncRNA ANRIL,urinary protein,and BDNF were influential factors for the occurrence of TE in NS patients(P<0.05);the ROC curve results showed that the area under the curve(AUC)of serum lncRNA ANRIL and BDNF in predict-ing the occurrence of TE in NS patients was 0.84 and 0.85,respectively,the AUC predicted by the combination of the two was 0.92,which was higher than that of the detection of lncRNA ANRIL and BDNF alone,with specificity of 83.05%and sensitivity of 88.24%.Conclusion The level of serum lncRNA ANRIL is increased and the level of BDNF is decreased in NS patients with TE,both have certain predictive value for the occurrence of TE in NS patients.
Nephrotic syndromeNon-coding RNA ANRILBrain derived neurotrophic factorThromboembolismPredictive value
万方数据
维普
