Serum levels of miR-218-5p and LASP1 in young patients with ischemic stroke and their application value
Objective To investigate the application value of the serum microRNA-218-5P(miR-218-5p),LIM and SH3 protein-1(LASP1)levels in young patients with ischemic stroke(IS).Methods A total of 96 young IS patients admitted to the Affiliated Hospi-tal of Shandong University of Traditional Chinese Medicine from June 2020 to June 2022 were selected as the IS group.All patients with IS were followed up for 3 months.According to the modified Rankin Scale(mRS)score,68 patients in the good prognosis group(mRS Score≤2)and 28 patients in the poor prognosis group(mRS Score>2)were grouped.A total of 96 healthy volunteers who under-went physical examination in our hospital during the same period were selected as control group.Serum miR-218-5p and LASP1 mRNA levels were detected using real-time fluorescence quantitative PCR(qRT-PCR).The relationship between the level of serum miR-218-5p and LASP1 mRNA was analyzed by Pearson correlation.The predictive value of serum miR-218-5p and LASP1 mRNA ex-pression levels on the prognosis of IS was analyzed by the receiver operating characteristic curve(ROC curve).Results Compared with the control group,the levels of white blood cell count,total cholesterol,triglyceride and low density lipoprotein cholesterol in IS group were significantly increased,and the levels of high density lipoprotein cholesterol were significantly decreased(P<0.05).Com-pared with the control group(1.03±0.11,1.01±0.11),the serum miR-218-5p level(0.88±0.09)in IS group was significantly decreased,and the serum LASP1 mRNA level(1.12±0.12)was significantly increased(P<0.05).Serum miR-218-5p was negatively correlated with LASP1 mRNA in IS patients(r=-0.73,P<0.001).Compared with the good prognosis group(0.94±0.10,1.05±0.11),the serum miR-218-5p(0.74±0.08)level was significantly decreased in the poor prognosis group,and the serum LASP1 mRNA level was significantly in-creased(1.28±0.13)(P<0.05).The ROC curve showed that the AUC of the combination of the two methods was larger than the miR-218-5p and LASP1 mRNA alone(Z=12.35,P<0.001;Z=6.60,P=0.010).Conclusion The serum miR-218-5p was low,and LASP1 mRNA was highin young IS patients,and the both were useful for evaluating the prognosis of the young patientswith IS.
StrokeBrain infarctionYouthMicroribonucleic acid-218-5pLIM and SH3 protein 1Prognosis
万方数据
维普
