Effect of BSA-CuS combined with PTT inhibiting oxidative stress and inflammatory response in diabetic foot ulcers
Objective To observe the effect of bovine serum albumin-CuS(BSA-CuS)combined with photothermal therapy(PTT)on oxidative stress and inflammatory response of mice with diabetic foot ulcers.Methods BSA-CuS nanomaterials were prepared by chemical method,and cultured StaphylococCuS aureus and Escherichia coli were treated from January to May 2023.The inhibition rate was calculated by plate coating method.DFU mice model was established by intraperitoneal injection of streptozotocin combined with wound mixed bacteria coating.BSA-CuS,PTT and BSA-CuS-PTT were given respectively.The content of malondialdehyde(MDA),su-peroxide dismutase(SOD),interleukin(IL)-1,IL-6 and tumor necrosis factor α(TNFα)in serum were detected by enzyme linked immu-nosorbent assay(ELISA).Western blotting(WB)was used to detect the protein expressions of Wingless type MMTV integration site family member 3A(Wnt3a),β-catenin,and glycogen synthase kinase-3 β(GSK-3β)in wound tissue.Results Compared with phos-phate buffer saline(PBS)+PTT group,the inhibition rates of Escherichia coli[(56.48±6.54)%vs.(6.48±1.37)%]and Staphylococcus aureus[(71.47±7.71)%vs.(6.56±1.38)%]in BSA-CuS+PTT group were significantly increased(P<0.05).Compared with BSA-CuS or PTT alone,the wound healing treated with BSA-CuS combined with PTT was significantly increased(P<0.05),and the levels of MDA,IL-1,IL-6 and TNF-α were significantly decreased(P<0.05).And meanwhile,SOD was significantly increased(P<0.05),the protein ex-pressions of Wnt3a,β-Catenin were significantly increased(P<0.05),while GSK-3β expression was significantly decreased(P<0.05)in DFU mice.Conclusion BSA-CuS combined with PTT could inhibit the oxidative stress and inflammatory reaction of DFU,which is beneficial to wound healing,and its mechanism may be related to the inactivation of Wnt signaling pathway.
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