Effect of matrine on apoptosis and autophagy of uterine fibroid cells
Objective To explore the effect of matrine on the apoptosis and autophagy of uterine fibroid cells.Methods The primary uterine fibroid cells were assigned into control group,low-dose group and high-dose group.Cells in the control group were cultured in conventional medium,cells in the low dose group and high dose group were treated with 0.5 g/L and 2 g/L matrine,respectively.Flow cytometry and polynucleotide chain break detection technology were used to detect the apoptosis level,the immunofluorescence tech-nology to detect autophagic spots in cells,transmission electron microscope to detect autophagosomes,and Western blotting to detect the levels of proteins related to apoptosis and autophagy.Results The relative apoptosis ratios of the control group,the low-dose group and the high-dose group were 1.00±0.02,2.97±0.13,and 3.18±0.17,respectively.Compared with the control group,the apoptosis of cells in the low-dose group and high-dose group increased(tlow-dose group vs.control group=26.07,Plow-dose group vs.control group=1.286×10-5;thigh-dose group vs.control group=22.73,Phigh-dose group vs.control group=2.219×10-5),while there was no statistically significant difference in cell apoptosis between the low-dose group and high-dose group(thigh-dose group vs.low-dose group=1.73,Phigh-dose group vs.low-dose group=0.158).The proportions of autophagic cells in the control group,the low-dose group and the high-dose group were 3.33±0.70,53.44±5.61,and 56.51±6.48,respectively.Compared with the con-trol group,the proportions of autophagic fluorescent spot cells increased in the low-dose group and high-dose group(tlow-dose group vs.control group=15.36,P low-dose group vs.control group=1.048×10-4;thigh-dose group vs.control group=14.12,Phigh-dose group vs.control group=1.458×10-4),while there was no statistically sig-nificant difference between the low-dose group and the high-dose group(thigh-dose group vs.Low-dose group=0.62,Phigh-dose group vs.Low-dose group=0.569).Conclu-sion Martine can enhance apoptosis and autophagy of uterine fibroid cells.
万方数据
维普
