Effect of galangin on vascular endothelial cell injury in atherosclerotic rats by regulating miR-124/STAT3 axis
Objective To investigate the effect of galangin on vascular endothelial cell injury in atherosclerosis(AS)rats by regulat-ing the micro RNA124(miR-124)/signal transducer and activator of transcription 3(STAT3)axis.Methods The study started from January 2023 and ended in May 2023.Primary cultured rat aortic endothelial cells were grouped into control group(without any treat-ment),model group(80 mg/L ox-LDL treatment),galangin group(50 μmol/L galangin+80 mg/L ox-LDL treatment),galangin+inhibitor NC group(transfected with inhibitor NC after treatment with 50 μmol/L galangin+80 mg/L ox-LDL),galangin+miR-124 inhibitor group(transfected with miR-124 inhibitor after treatment with 50 μmol/L galangin+80 mg/L ox-LDL).The dual luciferase gene reporter assay was applied to verify the targeting relationship between miR-124 and STAT3,real-time fluorescent quantitative reverse transcription polymerase chain reaction(qRT-PCR)to detect miR-124 expression,Western blotting to detect STAT3 protein expression,MTT meth-od to measure cell proliferation,flow cytometry to detect cell apoptosis,and enzyme-linked immunosorbent assay(ELISA)to detect the levels of inflammatory factors,nitric oxide(NO)and reactive oxygen species(ROS).Results MiR-124 targeted and regulated the STAT3 expression.Compared with the control group,the miR-124 level(1.00±0.00 vs.0.45±0.06)and D(λ)490 value(0.98±0.13 vs.0.44±0.05)in the model group were obviously decreased(P<0.05),while the apoptosis rate[(5.58±0.68)%vs.(15.83±2.06)%],STAT3 level(0.48±0.05 vs.1.08±0.13),and the levels of IL-1β[(12.05±1.17)mg/L vs.(22.42±2.36)mg/L],IL-6[(4.13±0.48)mg/L vs.(9.64±0.98)mg/L],TNF-α[(2.11±0.25)mg/L vs.(6.63±0.66)mg/L],NO[(22.26±2.33)μmol/L vs.(90.25±9.44)μmol/L],ROS[(32.93±3.52)μmol/L vs.(60.91±7.19)μmol/L]were obviously increased(P<0.05).Compared with the model group,the miR-124 level(0.45±0.06 vs.0.89±0.01)and D(λ)490 value(0.44±0.05 vs.0.84±0.09)in the galangin group were obviously increased(P<0.05),while the apoptosis rate[(15.83±2.06)%vs.(6.28±0.72)%],and the levels of STAT3(1.08±0.13)vs.(0.53±0.06),IL-1β[(22.42±2.36)mg/L vs.(16.92±1.93)mg/L[,IL-6[(9.64±0.98)mg/L vs.(5.42±0.53)mg/L],TNF-α[(6.63±0.66)mg/L vs.(3.57±0.37)mg/L],NO[(90.25±9.44)μmol/L vs.(34.84±4.10)μmol/L],and ROS[(60.91±7.19)μmol/L vs.(30.41±3.05)μmol/L]were obviously decreased(P<0.05).Down-regula-tion of miR-124 inhibited the improvement of galangin on vascular endothelial cell injury in AS rats.Conclusion Galangin can down-regulate the expression of STAT3 by up-regulating miR-124,thereby alleviating the injury of vascular endothelial cells in AS rats.
AtherosclerosisGalanginMicro RNA124Signal transducer and activator of transcription 3Vascular endothelial cells
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