Effect of propofol on lipopolysaccharide-induced adhesion,migration,invasion and inflammatory factors of MHCC97H cells and its relationship with NF-κB signaling pathway
Objective To investigate the relationship between propofol and lipopolysaccharide(LPS)-induced MHCC97H cell func-tion,inflammation level and nuclear factor-κB(NF-κB)expression.Methods The study period was from December 2021 to December 2022.Human MHCC97H cell lines were cultured in vitro and assigned into control group(equal volume of solvent),LPS group(1 mg/L LPS),experimental group(LPS+6.25 group,LPS+12.5 group,LPS+25 group,with 6.25,12.5 and 25 μmol/L propofol added to LPS group,respectively).Enzyme-linked immunosorbent assay and cell counting kit were used to detect the expression level of inflammatory factor interleukin-6(IL-6)and cell activity to screen out the optimal concentration of propofol for follow-up experiments.Then the cells were assigned into control group,LPS group,LPS+25 group and LPS+25+inhibitor group(LPS+25 group was combined with 10 μmol/L BAY 11-7082),which were treated for 24 h.The number of adherent cells was measured by cell adhesion assay,cell migration and in-vasion were detected by Transwell chamber assay,and epithelial-mesenchymal transition(EMT)and NF-κB pathway-related proteins expression levels were determined by Western blotting.Results According to cell viability and the expression of inflammatory factor IL-6,LPS+25 group was selected for subsequent experiments.The counts of cell adhesion,migration,invasion,and N-cadherin,Vimen-tin,fibronectin(FN),IκB kinase α(IKKα)and p-NF-κB p65 protein expression levels[(152.00±9.01)cells,(84.01±10.44)cells,(65.67±3.06)cells,0.46±0.02,0.47±0.03,0.99±0.02,1.03±0.02 and 0.95±0.05,respectively]in the LPS group were higher than those in the control group,while E-cadherin expression level(0.42±0.02)was lower than that in the control group(P<0.05).The LPS+25 group significantly reversed the changes of the above indicators in comparison with LPS group(P<0.05).LPS+25+inhibitor group reversed the changes of the above indicators after adding the NF-κB pathway inhibitor in comparison with LPS+25 group(P<0.05).Conclusion Propofol can inhibit the expression,adhesion,migration,invasion and EMT process of MHCC97H cells stimulated by LPS,which may be related to the inhibition of NF-κB pathway activity.
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