Serum circNfix expression in patients with chronic heart failure and its relationship with myocardial remodeling and cardiac function
Objective To analyze the expression level of circNfix in serum of patients with chronic heart failure and its relationship with myocardial remodeling and cardiac function.Methods From July 2020 to August 2021,105 patients with chronic heart failure who were treated in 3201 hospital were regarded as the study group,in addition,52 healthy people were selected as the control group.The expression of circNfix in serum was detected by real-time fluorescent quantitative PCR;echocardiography and two-dimensional pla-nar images were used to detect myocardial remodeling and cardiac function related indicators;the level of NT-proBNP was detected by automatic electrochemiluminescence immunoassay;the level of TNF-α was measured by radioimmunoassay;Pearson correlation was used to analyze the correlation between the expression of circNfix in serum and NT-proBNP,TNF-α,myocardial remodeling and cardi-ac function;Multivariate logistic regression was used to analyze risk factors affecting the development of chronic heart failure.Results The expression level of circNfix in grade I-Ⅱ in the study group was obviously lower than that in the control group(0.62±0.14 vs.1.01±0.23,P<0.05),the expression level in grade Ⅲ and grade Ⅳ was obviously lower than that in grade Ⅰ-Ⅱ(0.49±0.12,0.19±0.05 vs.0.62±0.14,P<0.05);the expression levels of NT-proBNP[(9.32±2.14)μg/L vs.(5.73±1.11)μg/L]and TNF-α[(35.27±10.24)ng/L vs.(24.94±6.58)ng/L]in grade Ⅰ-Ⅱ in the study group were obviously higher than the control group(P<0.05),the expression levels in grade Ⅲ[(12.90±2.78)μg/L,(54.84±12.29)ng/L]and grade Ⅳ[(15.76±3.06)μg/L,(92.93±16.69)ng/L]were obviously lower than those in grade Ⅰ-Ⅱ(P<0.05).LVEDD,LAD,LVPWD and LVMI in grade Ⅰ-Ⅱ in the study group were obviously higher than those in the control group(P<0.05),and those in grade Ⅳ were obviously higher than those in grade Ⅲ and grade Ⅰ-Ⅱ(P<0.05),LVRI,CO and LVEF were obviously lower than those in the control group(P<0.05),and those in grade Ⅳ were obviously lower than those in grade Ⅲand Ⅰ-Ⅱ(P<0.05).According to Pearson correlation analysis,the expression level of circNfix in serum of the study group was nega-tively related to NT-proBNP,TNF-α,LVEDD,LVPWD and LVMI(P<0.05),but positively correlated with CO and LVEF(P<0.05).Ac-cording to logistic regression analysis,low circNfix levels are a risk factor for the development of chronic heart failure(P<0.05).Conclu-sion The expression of circNfix in serum is decreased in patients with chronic heart failure,which is closely related to myocardial re-modeling and cardiac function.
Heart failureCircular RNACircNfixMyocardial remodelingCardiac function
万方数据
维普
