Optimisation of Microwave-Assisted Extraction of Poria Cocos Polysaccharides by Response Surface Methodology
Response surface method was used to optimize microwaves assisted extraction of polysaccharide from Poria sclerotium.On the basis of single factor experiment,Box-Behnken experiment design and response surface analysis were used to study the effects of solid-liquid ratio,pH value,microwave power,extraction time and extraction times on the extraction yield of Poria polysaccharide.The results showed that the optimal extraction process was as follows:liquid-solid ratio 28.3:1,pH6.8,microwave power 548W,extraction time 2.2min,extractiòn times 2 times.Under these conditions,the extraction yield was 3.04％,and the relative error between the extrac-tion yield and the predicted value was 3.06％was less than 5％.The extraction parameters obtained by this method are reliable and can provide technical reference for the industrial production of poria polysaccharide.
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