Research Progress in Tissue Culture and Organ de Novo Regeneration of Woody Plants
Tissue culture technology is an important means for the regeneration and reproduction of woody plants.This paper summarizes the research progress of tissue culture technology and molecular regulation mechanism of organ re-generation in woody plants.This scrutiny of research progress has made it clear that establishment of regeneration sys-tems for woody plants is closely related to internal factors(explant genotypes,physiological states,etc.)and external factors(plant hormones,culture conditions,etc.).Gene expression,hormone signaling pathways and other signaling pathways play key regulatory roles in the process of de novo regeneration of woody plant organs.The difficulty in induc-ing adventitious buds and roots can be a bottleneck in the regeneration of woody plants.In the future,regulatory mecha-nism of regeneration of woody plants should be further studied to improve the regeneration efficiency and quality of woody plants.