Effects of Rhizobial Inoculation and Nitrogen Application on Growth and Stress Tolerance of Intercropped Eucalyptus urophylla×E.grandis and Dalbergia odorifera
This study investigated the effects of inoculating rhizobacteria and applying nitrogen on the growth and stress resistance of Eucalyptus urophylla×E.grandis and Dalbergia odorifera plantations,aiming to provide a theoretical basis for their sustainable development.In the intercropping system,treatments included a control(CK),individual rhizobacterial inoculations(S1,S2,S3,S4,S5,S6),nitrogen application(N),mixed rhizobacterial inoculation(H),and a combination of mixed inoculation with nitrogen(HN).Physiological and growth indices of seedlings were measured.The results indicated that all inoculated rhizobacteria and nitrogen treatments significantly promoted the growth and stress tolerance of both E.urophylla×E.grandis and D.odorifera to varying degrees.The growth promotion was characterized by enhanced photosynthesis and transpiration,changes in root traits,increased biomass,and growth in height and ground diameter.Stress tolerance improvements were reflected in decreased malondialdehyde levels,increased soluble sugars,enhanced nitrate reductase activity,and elevated soluble protein content in E.urophylla×E.grandis.For growth and stress tolerance,N was the strongest promoter for E.urophylla×E.grandis,followed by HN and H.All single rhizobia inoculation treatments showed similar effects.For D.odorifera,HN was the most effective treatment for promoting growth and stress tolerance,followed by H and S3,with all other individual rhizobacterial treatments showed similar effects to nitrogen except S3.
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