Objective:To explore the mechanism of puerarin in the treatment of hepatocellular carcinoma(HCC)based on network pharmacology,bioinformatics and molecular docking techniques.Methods:The target of puerarin was obtained from TCMSP and other databases.HCC data sets were obtained from the National Center of Biotechnology Information(NCBI)-Integrated Gene Expression Database(GEO).Autophagy targets were obtained from HDDb database.The targets of the three were intersected and PPI networks were constructed.The intersection targets were analyzed for molecular docking,protein expression profile,clinical correlation,survival analysis,differential expression,ROC curve and KEGG pathway enrichment analysis.Results:A total of 5 intersection targets were obtained,and molecular docking showed that 3 proteins(RHEB,FAS,SERPINA1)were highly binding to puerarin.The protein expression profile indicated that RHEB was highly expressed in HCC,while FAS and SERPINA1 were low expressed.Clinical correlation analysis,survival analysis,differential expression analysis and ROC curve all supported that RHEB of puerarin treated HCC by acting on autophagy.KEGG pathway enrichment showed that RHEB was involved in the mTOR-autophagy pathway.Conclusion:Puerarin may play a role in the treatment of HCC by acting on RHEB,a gene associated with autophagy.
puerarinhepatocellular carcinoma(HCC)autophagyras homolog enriched in brain(RHEB)
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