Effects of Drought Stress on Growth and Physiological Indicators of Grafted Cucumber Seedlings
Taking cucumber'Zhongnong 18'as the experimental material,black seed pumpkin was used as the rootstock during the seedling stage for grafting and root replacement.The grafted and self rooted seedlings were subjected to drought stress,and the plant height,stem diameter,dry matter weight,chlorophyll content,peroxidase(POD)activity,superoxide dismutase(SOD)activity,catalase(CAT)activity,malondialdehyde(MDA)content,root activity,abscisic acid(ABA)content,and zein(ZR)content were measured.The effects of drought stress on the growth and development of grafted cucumber seedlings were studied,in order to provide reference for the study of cucumber stress physiology.The results showed that the growth and development of both grafted and self-rooted seedlings were affected under drought stress,manifested as a slow accumulation of plant height,stem diameter,and dry matter weight.Compared with self-rooted seedlings,grafted seedlings had higher plant height,stem diameter,and dry matter weight,reaching 17.54 cm,6.24 mm and 1.2 g after 9 days of treatment,respectively.Further analysis of physiological indicators revealed that drought stress had a certain degree of inhibition on both grafted and self-rooted cucumber seedlings,manifested as an increase in chlorophyll content,POD activity,SOD activity,CAT activity and endogenous hormone content in the early stage,followed by a decrease in the later stage.MDA content gradually increased,and the root activity of grafted seedlings first increased and then decreased,while the root activity of self-rooted seedlings gradually decreased.The physiological indicators of grafted seedlings were higher than those of self-rooted seedlings,except for MDA content.The highest peaks of chlorophyll content,POD activity,SOD activity,CAT activity,MDA content,root activity and endogenous hormone content were 2.588 mg·g-1,6.229 U·g-1·min-1,254.098 U·g-1,212.341 U·g-1·min-1,11.052 mmol·g-1,3.577 μmol·g-1,12.578 ng·g-1.In conclusion grafted seedlings had a strong ability to resist drought and were less affected.They still maintained normal growth under adverse conditions,while self-rooted seedlings had poor drought resistance.Therefore,the use of grafting significantly improved the drought resistance of seedlings,which was simple and effective and can be applied in arid and water deficient areas.
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万方数据
维普
