首页|姬松茸发酵培养料的理化性质及微生物群落研究

姬松茸发酵培养料的理化性质及微生物群落研究

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
以牛粪和玉米芯为主料进行发酵,采用高通量测序法,设置未发酵(C1)、第1次发酵完成(C2)、第2次发酵完成(C3)3个阶段,研究了不同发酵阶段培养料的理化性质及微生物群落演替的变化规律,以期探究姬松茸培养料隧道式发酵特性,优化隧道发酵技术.结果表明:姬松茸培养料发酵过程中含水率、pH、总碳和C/N不断下降,电导率和全氮含量呈先降低后升高的趋势.细菌群落中厚壁菌在整个时期均为优势菌门;放线菌与异常球菌在C2时期成为优势菌群;C3时期绿弯菌门的相对丰度显著上升.真菌群落中的子囊菌在整个培养料发酵过程中是最丰富的真菌门,相对丰度占比均在92.5％之上,其次为担子菌门.TN、pH、C/N、MC、TC和EC值均对发酵料中细菌群落结构有显著影响,其中TN对细菌群落的影响极为显著(P＜0.01);TC、C/N、MC和TN是影响发酵料中真菌群落的关键因素,其中TC和C/N的影响程度最为显著(P＜0.01).
Study on Physicochemical Properties and Dynamic Succession of Microbial Communities of Agaricus blazei Murill.Substrates Based on Tunnel Fermentation Technology
Cow dung and corncob were used as main materials for composting.The physicochemical properties and dynamic succession of microbial communities of culture materials at different composting stages were surveyed using metagenomic analysis.The experiment was divided into three stages,unfermented(C1),first composting completed(C2),and second composting completed(C3),in order to explore the characteristics of tunnel fermentation of Agaricus blazei Murrill.substrates and optimize the technology.The results showed that,the moisture content,pH,total carbon and C/N ratio decreased continuously during the composting.The electrical conductivity and total nitrogen content decreased first and then increased.Firmicutes was the dominant phylum in bacterial communities during the whole period.Actinobacteriota and Deinococcota became the dominant flora in C2 phase,the relative abundance of Chloroflexi increased significantly in C3 phase.Ascomycota was the most abundant fungal phylum throughout the composting process,with a relative abundance of more than 92.5％,followed by Basidiomycota.TN,pH,C/N ratio,MC,TC and EC had significant effects on bacterial community structure,and TN had a significant effect on the bacterial community(P＜0.01).TC,C/N ratio,MC and TN were the key factors affecting fungal communities,and the influence of TC and C/N ratio was extremely significant(P＜0.01).

Agaricus blazei Murill.tunnel fermentation technologyphysicochemical propertieshigh-throughput sequencingmicrobial community succession

赵玉阳、冯璠、郝刘斌、孙悦、郑素月、王春霞

展开 >

河北工程大学园林与生态工程学院,河北邯郸 056038

姬松茸 隧道式发酵技术 理化性质 高通量测序 微生物群落演替

河北省高等学校科学技术研究资助项目邯郸市科学技术研究与发展计划资助项目河北省农业科技成果转化资助项目

QN202121021422012326202360101010014

2024

10.11937/bfyy.20233826

北方园艺
黑龙江省农业科学院 黑龙江省园艺学会 黑龙江省农业科学院编辑出版中心

北方园艺

CSTPCD北大核心
影响因子：0.506
ISSN：1001-0009
年,卷(期)：2024.(9)
  • 30