Optimization of the Best Formula for Tissue Culture of Ornamental Miniature Orchid
The F1 test-tube seedlings of Cymbidium hybridium 'Dafeng'×C.serratum var.goeringii'Taijishengmei'were used as the test materials.Different organic substances were added to the medium to screen the optimal growth medium.On this basis,different pigments were added to the medium to study their effects on plant growth and ornamental effects,in order to provide reference for the study of ornamental application of orchid microculture.The results showed that in the medium of 1/2MS+IBA 0.5 mg·L-1+6-BA 2 mg·L-1+carbon powder 0.5 g·L-1,added 75 mL·L-1 coconut juice was benefited to the increased of leaf length,leaf width,plant height and fresh weight of orchid plantlets in vitro,and had the best effect on plant growth.In 1/2MS+IBA 0.5 mg·L-1+6-BA 2 mg·L-1+carbon powder 0.5 g·L-1+coconut juice 75 mL·L-1 medium added different concentrations of edible pigment monascus red,sodium copper chlorophyllin,gardenia blue,after 121 ℃ high temperature sterilization medium color was changed.The plants grew well in the color medium with the concentration of sodium copper chlorophyllin of 533.3 mg·L-1,and the overall ornamental effect of the medium was the best.
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