Mechanism of Nitric Oxide Signaling Pathway Regulating AsA-GSH Circulation of Pepper Under High Temperature Stress
Taking'Xinjianjiao 22'pepper as the experimental material,using the method of spraying different concentrations of exogenous NO donor SNP,NO synthase inhibitor,nitrate reductase inhibitor and NO scavenger under high temperature stress(38±1)℃,the effects of endogenous NO cont ent,ROS metabolism,key substances content,key enzyme activity and gene expression in AsA-GSH circulatory system of different treatments were studied,in order to reveal the mechanism by which the NO signaling pathway regulates the AsA-GSH cycle metabolism of pepper under high-temperature stress.The results showed that high temperature stress could significantly increased the endogenous NO content in pepper,while NO scavengers and enzyme inhibitors could significantly reduce the endogenous NO content.Adding appropriate concentration of exogenous NO could effectively increase the enzyme activities of ascorbate peroxidase(APX),monodehydroascorbate reductase(MDHAR),dehydroascorbate reductase(DHAR)and glutathione reductase(GR),up-regulate the expression of MDHAR,DHAR and GR genes,and increase the levels of AsA,dehydroascorbic acid(DHA),GSH and oxidized glutathione(GSSG).Adding NO scavenger and synthesis inhibitor would reverse the above changes.The results showed that NO signaling pathway played an important role in the response of pepper to high temperature stress,and NO signaling molecules could regulate the expression and post-translation modification of key genes in AsA-GSH circulation system of pepper,effectively enhance the activity of key enzymes,and increase the ratio of reduced state to oxidized state in AsA-GSH circulation,thus maintaining the balance of ROS in vivo.Supplementing appropriate exogenous NO could effectively improve the resistance of pepper to high temperature stress and enhance its heat resistance.
pepperhigh temperature stressNOAsA-GSH circulatory
NETL
NSTL
万方数据
