Research on Rooting Technology of'Benihoppe'Strawberry Sugar-free Tissue Culture and Screening of Transplanting Substrate
Taking'Benihoppe'strawberry as the test material,the rooting technology of'Benihoppe'strawberry sugar-free tissue culture was studied by using the method of sugar-free tissue culture,in order to provide reference for strawberry production practice.The results showed that the best rooting medium for sugar-free tissue culture was 1/2MS+0.4 mg·L-1 IBA,the best rooting medium for traditional tissue culture was 1/2MS+0.5 mg·L-1 IB A,the strawberry sugar-free tissue culture seedlings grew most robustly when the substrate was pure vermiculite as the substrate,the light duration was 12 hours,and the CO2 ventilation time was 3 days,and the survival rate of strawberry sugar-free tissue culture seedlings was 100％under the ratio of peat-soil∶vermiculite∶perlite=3∶1∶1.
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