Optimization of Reflux Extraction Process for Three Alkaloids From Zanthoxylum nitidum by Response Surface Method
Taking Zanthoxylum nitidum as the experimental material,the effects of reflux extraction time,extraction solvent concentration and solid-liquid ratio on the extraction rate of nitidine chloride,chelerythrine and sanguinarine were studied by response surface method,in order to obtain the optimal reflux extraction process of the three alkaloids,and provide reference for further development and utilization of alkaloids in Zanthoxylum nitidum.The results showed that the optimal reflux extraction condition of the three alkaloidsas follows,extraction time was 2.4 hours per time(repeated twice),extraction solvent was 67％ethanol,solid-liquid ratio was 1∶20 g·mL-1,and the yield of nitidine chloride,chelerythrine and sanguinarine were 0.381％,0.560％,0.058％,respectively.The results were closed to the predicted values of response surface method,it indicated that the three alkaloids response surface regression models fitted in this study were reliable.After optimization,the yield of nitidine chloride,chelerythrine and sanguinarine were increased by 14％,95％and 23％respectively,and the optimized extraction efficiency was high,which could be used for the preparation of three alkaloids in Zanthoxylum nitidum.
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