Study on the Enrichment of Selenium in Hericium erinaceus Mycelium
Four Hericium erinaceus was used as the starting strain,and plate and liquid fermentation culture methods were used.The effects of sodium selenite and selenium yeast on the growth of four species of Hericium erinaceus mycelium,compared the growth rate,selenium content,protein level,polysaccharide concentration,total phenol content,and antioxidant activity of the aqueous extract enriched with selenium-enriched mycelium with different concentrations of selenium,in order to provide reference for the development and application of selenium enrichment functional food of Hericium erinaceus.The results showed that among the four strains of'Hericium erinaceus 911''Hericium erinaceus 920''Hericium erinaceus king''Hericium erinaceus3008''Hericium erinaceus 911'had stronger selenium-enriched ability and the highest selenium content in yeast selenium culture.Selenium could increase the content of protein,polysaccharide,total phenol and other main active substances in mycelium of Hericium erinaceus.The highest scavenging rates of hydroxyl free radical,superoxide anion free radical and 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical were 67.53%,39.27%and 74.05%,respectively,when the concentration of the water extract of selenium-rich mycelium of'Hericium erinaceus 911'was 2 mg·mL-1,which were higher than those of control.