Metabolomics Analysis of the Effects of Organic Fertilizers on the Growth and Development of Astragalus membranaceus
Taking biennial Astragalus membranaceus as the test material,different concentrations of'Yuantaifeng'organic fertilizer treatment and widely targeted metabolomics techniques were used,the optimal concentration of organic fertilizer for root growth was studied,and the optimal organic fertilizer concentration treatment(OF)and no fertilizer treatment(CK)were compared by analyzing the differences in metabolite types and contents,and the KEGG enrichment pathway between groups were explored,in order to provide reference for further research on its medicinal value and artificial cultivation and breeding improvement.The results showed that the application of 6 000 kg·hm-2 organic fertilizer could effectively improve the agronomic traits of A.membranaceus,such as root length,root diameter,dry weight,and fresh weight.Metabolomics analysis revealed that a total of 11 major categories,including flavonoids,phenolic acids,lipids,amino acids,and their derivatives,were identified in the root system of Astragalus membranaceus,with 1 317 metabolites identified.Multivariate statistical analysis showed that there were significant differences in secondary metabolites between treatments,mainly including 250 substances such as flavonoids(147),phenolic acids(32),terpenes(20),etc.Among them,2-hydroxyoleanolic acid,3-hydroxyglycyrrhizic acid,and cochinchinchinanin A were the main differential substances.Compared with CK,most differential metabolites under OF treatment were upregulated and mainly composed of flavonoids.Meanwhile,KEGG analysis revealed that differential metabolites were mainly enriched in three flavonoid biosynthesis pathways,isoflavone biosynthesis,flavonoid and flavonol biosynthesis,and flavonoid biosynthesis.In summary,there were significant differences in root growth of Astragalus membranaceus under different organic fertilizer applications,with the best performance observed at an organic fertilizer concentration of 6 000 kg·hm-2.At the optimal fertilization concentration,most of the secondary metabolites in the roots of Astragalus membranaceus were upregulated,with flavonoids being the most significant.
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