Identification and Molecular Evolutionary Analysis of Jasmine Virus T Infecting Jasmine in Gansu Province
Eight jasmine leaves showing yellow ring were used as experimental material.And the pathogen causing jasmine virus disease and the nucleic acid sequence,genetic diversity of jasmine virus T(JaVT)were analyzed by RT-PCR,in order to provide reference for the prevention and treatment of jasmine virus disease.The results showed that JaVT was isolated from 4 samples,and 4 CP gene sequences of JaVT were amplified.The nucleotide sequence identity among the CP genes of the four JaVT isolates was 92.20%-95.20%,and the nucleotide sequence identity between the CP genes of the four JaVT isolates was 91.40%-94.00%with the other 4 JaVT CP genes published in NCBI.Phylogenetic analysis based on the full-length sequences of 8 JaVT CP genes.The results showed that the four JaVT isolates in this study were clustered with JaVT-MZ962345 from China.Three recombination events were detected in 8 JaVT full-length CP gene sequences.
jasminejasmine T virusvirus detectionCPmolecular evolutionary analysis