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侵染甘肃茉莉的茉莉T病毒鉴定及分子进化分析

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以表现黄色环纹症状的8份茉莉叶片为试材,采用RT-PCR方法,研究了待测茉莉的病毒种类及茉莉T病毒(jasmine virus T,JaVT)外壳蛋白(coat protein,CP)基因序列遗传多样性,以期为茉莉病毒病的防治提供参考依据.结果表明:4份样品中检测到JaVT,并扩增出4条JaVT的外壳蛋白(CP)基因序列.4个JaVT分离物CP基因间核苷酸序列的一致性为92.20%~95.20%,与NCBI已发表的4个JaVT CP基因核苷酸序列一致性为91.40%~94.00%.基于8个JaVT CP基因全长序列进行系统发育分析,结果表明该研究中的4个JaVT分离物与来自中国的JaVT-MZ962345聚集在一起.8条JaVT全长CP基因序列中检测到3个重组事件.这是甘肃省首次报道茉莉T病毒.
Identification and Molecular Evolutionary Analysis of Jasmine Virus T Infecting Jasmine in Gansu Province
Eight jasmine leaves showing yellow ring were used as experimental material.And the pathogen causing jasmine virus disease and the nucleic acid sequence,genetic diversity of jasmine virus T(JaVT)were analyzed by RT-PCR,in order to provide reference for the prevention and treatment of jasmine virus disease.The results showed that JaVT was isolated from 4 samples,and 4 CP gene sequences of JaVT were amplified.The nucleotide sequence identity among the CP genes of the four JaVT isolates was 92.20%-95.20%,and the nucleotide sequence identity between the CP genes of the four JaVT isolates was 91.40%-94.00%with the other 4 JaVT CP genes published in NCBI.Phylogenetic analysis based on the full-length sequences of 8 JaVT CP genes.The results showed that the four JaVT isolates in this study were clustered with JaVT-MZ962345 from China.Three recombination events were detected in 8 JaVT full-length CP gene sequences.

jasminejasmine T virusvirus detectionCPmolecular evolutionary analysis

陈雅寒、路云爽、何志兰、王骆凡

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甘肃农业大学植物保护学院,甘肃兰州 730070

茉莉 JaVT 病毒检测 CP 分子进化分析

甘肃农业大学人才启动金资助项目国家自然科学基金资助项目甘肃省自然科学基金资助项目甘肃省高等学校创新基金资助项目

GAU-KYQD-2019-223216062821JR7RA8202021B-117

2024

北方园艺
黑龙江省农业科学院 黑龙江省园艺学会 黑龙江省农业科学院编辑出版中心

北方园艺

CSTPCD北大核心
影响因子:0.506
ISSN:1001-0009
年,卷(期):2024.(17)
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