The grape variety of'Summer Black'was used as experimental material.The response surface test design was carried out on the basis of single factor test for the melatonin concentration,soaking time and the thickness of PE bag in the storage of grape stem.With the comprehensive index as the response value,the preservation process of grape stem was optimized at room temperature,and the influence of the best preservation process on the preservation effect of grape stem was studied,in order to provide reference for the storage and preservation of grape at room temperature.The results showed that the optimal storage conditions of grape stem by response surface method were melatonin concentration of 0.450 mmol·L-1,soaking time of 23.00 minutes and PE bag thickness of 0.060 mm.Compared with blank(control)treatment,the optimal preservation process effectively delayed the increase of weight loss rate and browning index of'Summer Black'grape stem during storage,inhibited the increase of malondialdehyde content,reduced the activities of PPO and POD during the water loss of grape stem,and then slowed down the browning of grape stem.Therefore,melatonin combined with PE packaging treatment has a significant effect on the'green protection'of grape stem,which can maintain the quality of fruit stem and extend the storage period.
melatoninPE bagresponse surface methodstemprotect green