Taking the embryonic roots of Clematis courtoisii as the experimental material,embryogenic callus was induced.Hormone ratio and radiometric analysis methods were used to study the effects of hormone ratio and radiometric analysis on callus differentiation,in order to provide reference for the research on tissue culture and mutation germplasm innovation of Clematis courtoisii.The results showed that 6-BA 2 mg·L-1+NAA 0.05 mg·L-1 was the optimal medium for inducing callus differentiation.By using different doses of 137 Cs-γ rays(0,30,60,90,120,150,180 Gy)for radiation treatment,the radiation dose of 60 Gy had the least impact on callus differentiation rate,and 120 Gy was the semi lethal dose of Clematis courtoisii.The appropriate dose range for 137 Cs-γ radiation in the callus tissue of Clematis courtoisii was 60-90 Gy.
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